High-resolution ion mobility spectrometry-mass spectrometry of isomeric/isobaric ribonucleotide variants.

ABSTRACT: In this report, we explored the benefits of cyclic ion mobility (cIM) mass spectrometry in the analysis of isomeric post-transcriptional modifications of RNA. Standard methyl-cytidine samples were initially utilized to test the ability to correctly distinguish different structures sharing the same elemental composition and thus molecular mass. Analyzed individually, the analytes displayed characteristic arrival times (t_D ) determined by the different positions of the modifying methyl groups onto the common cytidine scaffold. Analyzed in mixture, the widths of the respective signals resulted in significant overlap that initially prevented their resolution on the t_D scale. The separation of the four isomers was achieved by increasing the number of passes through the cIM

SUBMITTER: Kenderdine T 

PROVIDER: S-EPMC8363168 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature