Fiber-optic label-free biosensor for SARS-CoV-2 spike protein detection using biofunctionalized long-period fiber grating.

ABSTRACT: With COVID-19 widespread worldwide, people are still struggling to develop faster and more accurate diagnostic methods. Here we demonstrated the label-free detection of SARS-CoV-2 spike protein by employing a SARS-CoV-2 spike antibody-conjugated phase-shifted long-period fiber grating (PS-LPFG) inscribed with a CO₂ laser. At a specific cladding mode, the wavelength separation (λ_D) between the two split dips of a PS-LPFG varies with the external refractive index, although it is virtually insensitive to ambient temperature variations. To detect SARS-CoV-2 spike protein, SARS-CoV-2 spike antibodies were immobilized on the fiber surface of the fabricated PS-LPFG functionalized through chemical modification. When exposed to SARS-CoV-2 spike protein with different concentra

SUBMITTER: Lee SL 

PROVIDER: S-EPMC8363218 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature