Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production.

ABSTRACT: Paecilomyces variotii xylanase was, produced in stirred tank bioreactor with yield of 760 U/mL and purified using 70% ammonium sulfate precipitation and ultra-filtration causing 3.29-fold purification with 34.47% activity recovery. The enzyme purity was analyzed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirming its monomeric nature as single band at 32 KDa. Zymography showed xylan hydrolysis activity at the same band. The purified enzyme had optimum activity at 60 °C and pH 5.0. The pH stability range was 5-9 and the temperature stability was up 70 °C. Fe²⁺and Fe³⁺ exhibited inhibition of xylanase enzyme while Cu²⁺, Ca²⁺, Mg²⁺ and Mn²⁺ stimulated its activity. Mercaptoethanol stimulated its a

SUBMITTER: Abdella A 

PROVIDER: S-EPMC8363652 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature