First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana.

ABSTRACT:

Background

Poxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former having zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, with no prior use of molecular tools to diagnose and characterize the pathogens.

Methods

A high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in skin biopsy and skin scab samples from four cattle, one sheep, and one goat. Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes.

Result

SUBMITTER: Modise BM 

PROVIDER: S-EPMC8364013 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature