Generation of equine enteroids and enteroid-derived 2D monolayers that are responsive to microbial mimics.

ABSTRACT: Enteroid cultures are three-dimensional in vitro models that reflect the cellular composition and architecture of the small intestine. One limitation with the enteroid conformation is the enclosed lumen, making it difficult to expose the apical surface of the epithelium to experimental treatments. The present study was therefore conducted to generate cultures of equine enteroids and to develop methods for culture of enteroid-derived cells on a two-dimensional plane, enabling easy access to the apical surface of the epithelium. Equine enteroids were established from small intestinal crypts within 7-9 days of culture. Transcriptional analysis of cell type markers confirmed the presence of enterocytes, stem-, Paneth-, proliferative-, enteroendocrine-, goblet- and tuft cells. This cellular com

SUBMITTER: Hellman S 

PROVIDER: S-EPMC8364015 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature