PI3K-AKT, JAK2-STAT3 pathways and cell-cell contact regulate maspin subcellular localization.

ABSTRACT:

Background

Maspin (SERPINB5) is a potential tumor suppressor gene with pleiotropic biological activities, including regulation of cell proliferation, death, adhesion, migration and gene expression. Several studies indicate that nuclear localization is essential for maspin tumor suppression activity. We have previously shown that the EGFR activation leads to maspin nuclear localization in MCF-10A cells. The present study investigated which EGFR downstream signaling molecules are involved in maspin nuclear localization and explored a possible role of cell-cell contact in this process.

Methods

MCF-10A cells were treated with pharmacological inhibitors against EGFR downstream pathways followed by EGF treatment. Maspin subcellular localization was determined by immunofluorescence

SUBMITTER: Longhi MT 

PROVIDER: S-EPMC8364028 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature