Dock2 affects the host susceptibility to Citrobacter rodentium infection through regulating gut microbiota.

ABSTRACT:

Background

Dysregulated gut microbiota is one of major pathogenic factors in the development of colitis. Dock2 acts as a guanine nucleotide exchange factor (GEF) and activates small G protein RAC1. Our previous study showed that, compared to wild type (WT) mice, Dock2^-/- mice were more susceptible to colitis induced by Citrobacter rodentium infection. However, it is not clear whether gut microbiota affects the host susceptibility to enteric bacterial infection in Dock2^-/- mice.

Results

In this study, we demonstrated that Dock2 regulated the gut microbiota and affected the host susceptibility to C. rodentium infection by co-housing, fecal microbiota transfer and antibiotic treatment methods. Microbiota analysis by 16 S rRNA gene sequencing showed that Do

SUBMITTER: Xie Y 

PROVIDER: S-EPMC8364044 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature