Synaptic Components, Function and Modulation Characterized by GCaMP6f Ca²⁺ Imaging in Mouse Cholinergic Myenteric Ganglion Neurons.

ABSTRACT: The peristaltic contraction and relaxation of intestinal circular and longitudinal smooth muscles is controlled by synaptic circuit elements that impinge upon phenotypically diverse neurons in the myenteric plexus. While electrophysiological studies provide useful information concerning the properties of such synaptic circuits, they typically involve tissue disruption and do not correlate circuit activity with biochemically defined neuronal phenotypes. To overcome these limitations, mice were engineered to express the sensitive, fast Ca²⁺ indicator GCaMP6f selectively in neurons that express the acetylcholine (ACh) biosynthetic enzyme choline acetyltransfarse (ChAT) thereby allowing rapid activity-driven changes in Ca²⁺ fluorescence to be observed without disrupting i

SUBMITTER: Margiotta JF 

PROVIDER: S-EPMC8365335 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature