Fast and Efficient Genome Editing of Human FOXP3⁺ Regulatory T Cells.

ABSTRACT: FOXP3⁺ regulatory T cells (Tregs) are central for maintaining peripheral tolerance and immune homeostasis. Because of their immunosuppressive characteristics, Tregs are a potential therapeutic target in various diseases such as autoimmunity, transplantation and infectious diseases like COVID-19. Numerous studies are currently exploring the potential of adoptive Treg therapy in different disease settings and novel genome editing techniques like CRISPR/Cas will likely widen possibilities to strengthen its efficacy. However, robust and expeditious protocols for genome editing of human Tregs are limited. Here, we describe a rapid and effective protocol for reaching high genome editing efficiencies in human Tregs without compromising cell integrity, suitable for potential therapeutic

SUBMITTER: Van Zeebroeck L 

PROVIDER: S-EPMC8365355 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature