Bacterial surface properties influence the activity of the TAT-RasGAP_317-326 antimicrobial peptide.

ABSTRACT: Antibiotic resistance is an increasing threat for public health, underscoring the need for new antibacterial agents. Antimicrobial peptides (AMPs) represent an alternative to classical antibiotics. TAT-RasGAP_317-326 is a recently described AMP effective against a broad range of bacteria, but little is known about the conditions that may influence its activity. Using RNA-sequencing and screening of mutant libraries, we show that Escherichia coli and Pseudomonas aeruginosa respond to TAT-RasGAP_317-326 by regulating metabolic and stress response pathways, possibly implicating two-component systems. Our results also indicate that bacterial surface properties, in particular integrity of the lipopolysaccharide layer, influence peptide binding and entry. Finall

SUBMITTER: Georgieva M 

PROVIDER: S-EPMC8365389 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature