CircCD151 promotes GLI2 expression by regulating miR‑30d‑5p and enhancing proliferation, invasion and stemness of lung cancer.

ABSTRACT: To investigate the changes of circular (circ)RNA circCD151 expression in lung cancer tissues and cells and its effects on proliferation, migration and invasion of lung cancer cells. The relative expression levels of circCD151 in lung cancer tissues and lung cancer cells (A549 and NCI‑H292) were determined by reverse transcription‑quantitative PCR. The effects of silencing or upregulation of circCD151 on the activity and clonal forming ability of A549 and NCI‑H292 cells were detected by CCK‑8 and cloning formation experiments. Transwell invasion assay detected the effects of silencing or upregulation of circCD151 on the migration and invasion ability of A549 and NCI‑H292 cells. The regulatory effect of circCD151 on miR‑30d‑5p was detected by dual luciferase reporter gene. The relative expre

SUBMITTER: Zhao L 

PROVIDER: S-EPMC8365425 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature