Protease-activated receptor-2 promotes osteogenesis in skeletal mesenchymal stem cells at the expense of adipogenesis: Involvement of interleukin-6.

ABSTRACT: Bone marrow mesenchymal stem cells (MSCs) give rise to osteoblasts and adipocytes, with an inverse relationship between the two. The MSCs from protease-activated receptor-2 knockout (PAR₂ KO) mice have a reduced capacity to generate osteoblasts. Here we describe the observation that PAR₂ KO osteoblastic cultures generate more adipocytes than wildtype (WT) cultures. Osteoblasts from PAR₂ KO mice expressed lower levels of osteoblastic genes (Runx2, Col1a1 and Bglap), and higher levels of the adipocytic gene Pparg than WT osteoblasts. Bone marrow stromal cells from PAR₂ KO mice generated fewer osteoblastic colonies (assessed by staining for alkaline phosphatase activity and mineral deposition) and more adipocytic (Oil Red

SUBMITTER: Sanaei R 

PROVIDER: S-EPMC8365448 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature