A genotype-phenotype screening system using conditionally immortalized immature dendritic cells.
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ABSTRACT:
SUBMITTER: Zhao L
PROVIDER: S-EPMC8365513 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
A genotype-phenotype screening system using conditionally immortalized immature dendritic cells.
Zhao Liwei L Liu Peng P Xie Wei W Zhang Shuai S Thieme Sebastian S Zitvogel Laurence L Kroemer Guido G Kepp Oliver O
STAR protocols 20210812 3
Here, we describe a protocol for CRISPR/Cas9-mediated gene knockout in conditionally immortalized immature dendritic cells (DCs), which can be limitlessly expanded before differentiation. This facilitates the genetic screening of DC functions <i>in vitro</i> including assessment of phagocytosis, cytokine production, expression of co-stimulatory or co-inhibitory molecules, and antigen presentation, as well as evaluation of the capacity to elicit anticancer immune responses <i>in vivo</i>. Altoget ...[more]
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