Generation of hiPSC-Derived Functional Dopaminergic Neurons in Alginate-Based 3D Culture.

ABSTRACT: Human induced pluripotent stem cells (hiPSCs) represent an unlimited cell source for the generation of patient-specific dopaminergic (DA) neurons, overcoming the hurdle of restricted accessibility to disease-affected tissue for mechanistic studies on Parkinson's disease (PD). However, the complexity of the human brain is not fully recapitulated by existing monolayer culture methods. Neurons differentiated in a three dimensional (3D) in vitro culture system might better mimic the in vivo cellular environment for basic mechanistic studies and represent better predictors of drug responses in vivo. In this work we established a new in vitro cell culture system based on the microencapsulation of hiPSCs in small alginate/fibronectin beads and their differentiation to

SUBMITTER: Gilmozzi V 

PROVIDER: S-EPMC8365765 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature