DDX52 knockdown inhibits the growth of prostate cancer cells by regulating c-Myc signaling.

ABSTRACT:

Background

DDX52 is a type of DEAD/H box RNA helicase that was identified as a novel prostate cancer (PCa) genetic locus and possible causal gene in a European large-scale transcriptome-wide association study. However, the functions of DDX52 in PCa remain undetermined. The c-Myc oncogene plays a crucial role in the development of PCa, but the factors that regulate the activity of c-Myc in PCa are still unknown.

Methods

We determined DDX52 protein levels in PCa tissues using immunohistochemistry (IHC). DDX52 expression and survival outcomes in other PCa cohorts were examined using bioinformatics analysis. The inhibition of DDX52 via RNA interference with shRNA was used to clarify the effects of DDX52 on PCa cell growth in vitro and in vivo. Gene set enrichment analysis and RN

SUBMITTER: Yu W 

PROVIDER: S-EPMC8365980 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature