Mutational profiling in acute lymphoblastic leukemia by RNA sequencing and chromosomal genomic array testing.

ABSTRACT:

Background

Comprehensive molecular and cytogenetic profiling of acute lymphoblastic leukemia (ALL) is important and critical to the current standard of care for patients with B-acute lymphoblastic leukemia (B-ALL). Here we propose a rapid process for detecting gene fusions whereby FusionPlex RNA next-generation sequencing (NGS) and DNA chromosome genomic array testing (CGAT) are combined for a more efficient approach in the management of patients with B-ALL.

Methods

We performed RNA NGS and CGAT on 28 B-ALL samples and, in four patients, compared fixed cell pellets to paired cryo-preserved samples as a starting material to further assess the utility of cytogenetic fixed pellets for gene expression analysis.

Results

Among the fixed specimens, when using alternative tec

SUBMITTER: Yeung C 

PROVIDER: S-EPMC8366081 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature