Validation of a Host Gene Expression Test for Bacterial/Viral Discrimination in Immunocompromised Hosts.

ABSTRACT:

Background

Host gene expression has emerged as a complementary strategy to pathogen detection tests for the discrimination of bacterial and viral infection. The impact of immunocompromise on host-response tests remains unknown. We evaluated a host-response test discriminating bacterial, viral, and noninfectious conditions in immunocompromised subjects.

Methods

An 81-gene signature was measured using real-time-polymerase chain reaction in subjects with immunocompromise (chemotherapy, solid-organ transplant, immunomodulatory agents, AIDS) with bacterial infection, viral infection, or noninfectious illness. A regularized logistic regression model trained in immunocompetent subjects was used to estimate the likelihood of each class in immunocompromised subjects.

Results

A

SUBMITTER: Mahle RE 

PROVIDER: S-EPMC8366815 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature