Organelle-specific phase contrast microscopy enables gentle monitoring and analysis of mitochondrial network dynamics.

ABSTRACT: Mitochondria are delicate organelles that play a key role in cell fate. Current research methods rely on fluorescence labeling that introduces stress due to photobleaching and phototoxicity. Here we propose a new, gentle method to study mitochondrial dynamics, where organelle-specific three-dimensional information is obtained in a label-free manner at high resolution, high specificity, and without detrimental effects associated with staining. A mitochondria cleavage experiment demonstrates that not only do the label-free mitochondria-specific images have the required resolution and precision, but also fairly include all cells and mitochondria in downstream morphological analysis, while fluorescence images omit dim cells and mitochondria. The robustness of the method was tested on samples o

SUBMITTER: Guo S 

PROVIDER: S-EPMC8367278 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature