Ontology highlight
ABSTRACT:
SUBMITTER: Bera SC
PROVIDER: S-EPMC8367775 | biostudies-literature | 2021 Aug
REPOSITORIES: biostudies-literature
Bera Subhas Chandra SC Seifert Mona M Kirchdoerfer Robert N RN van Nies Pauline P Wubulikasimu Yibulayin Y Quack Salina S Papini Flávia S FS Arnold Jamie J JJ Canard Bruno B Cameron Craig E CE Depken Martin M Dulin David D
Cell reports 20210817 9
Coronaviruses have evolved elaborate multisubunit machines to replicate and transcribe their genomes. Central to these machines are the RNA-dependent RNA polymerase subunit (nsp12) and its intimately associated cofactors (nsp7 and nsp8). We use a high-throughput magnetic-tweezers approach to develop a mechanochemical description of this core polymerase. The core polymerase exists in at least three catalytically distinct conformations, one being kinetically consistent with incorporation of incorr ...[more]