Protective effect of LNA-anti-miR-132 therapy on liver fibrosis in mice.

ABSTRACT: microRNAs (miRs) are small regulatory RNAs that are frequently deregulated in liver disease. Liver fibrosis is characterized by excessive scarring caused by chronic inflammatory processes. In this study, we determined the functional role of miR-132 using a locked nucleic acid (LNA)-anti-miR approach in liver fibrosis. A significant induction in miR-132 levels was found in mice treated with CCl₄ and in patients with fibrosis/cirrhosis. Inhibition of miR-132 in mice with LNA-anti-miR-132 caused decreases in CCl₄-induced fibrogenesis and inflammatory phenotype. An attenuation in collagen fibers, α SMA, MCP1, IL-1β, and Cox2 was found in LNA-anti-miR-132-treated mice. CCl₄ treatment increased caspase 3 activity and extracellular vesicles (EVs) in control but no

SUBMITTER: Momen-Heravi F 

PROVIDER: S-EPMC8368790 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature