Discrepancy between PCR based SARS-CoV-2 tests suggests the need to re-evaluate diagnostic assays.

ABSTRACT:

Objective

We investigated the discrepancy between clinical and PCR-based diagnosis of COVID-19. We compared results of ten patients with mild to severe COVID-19. Respiratory samples from all cases were tested on the Roche SARS-CoV-2 (Cobas) assay, Filmarray RP2.1 (bioMereiux) and TaqPath™ COVID19 (Thermofisher) PCR assays.

Results

Laboratory records of ten patients with mild to severe COVID-19 were examined. Initially, respiratory samples from the patients were tested as negative on the SARS-CoV-2 Roche^® assay. Further investigation using the BIOFIRE^® Filmarray RP2.1 assay identified SARS-CoV-2 as the pathogen in all ten cases. To investigate possible discrepancies between PCR assays, additional testing was conducted using the TaqPath™ COVID19 PCR. Eigh

SUBMITTER: Mushtaq MZ 

PROVIDER: S-EPMC8369441 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature