Transcriptomic analysis to elucidate the effects of high stocking density on grass carp (Ctenopharyngodon idella).

ABSTRACT:

Background

Grass carp (Ctenopharyngodon idella) is one of the most widely cultivated fishes in China. High stocking density can reportedly affect fish growth and immunity. Herein we performed PacBio long-read single-molecule real-time (SMRT) sequencing and Illumina RNA sequencing to evaluate the effects of high stocking density on grass carp transcriptome.

Results

SMRT sequencing led to the identification of 33,773 genes (14,946 known and 18,827 new genes). From the structure analysis, 8,009 genes were detected with alternative splicing events, 10,219 genes showed alternative polyadenylation sites and 15,521 long noncoding RNAs. Further, 1,235, 962, and 213 differentially expressed genes (DEGs) were identified in the intestine, muscle, and brain tissues, respectively. We per

SUBMITTER: He Y 

PROVIDER: S-EPMC8369720 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature