Time-dependent effect of 1,6-hexanediol on biomolecular condensates and 3D chromatin organization.

ABSTRACT:

Background

Biomolecular condensates have been implicated in multiple cellular processes. However, the global role played by condensates in 3D chromatin organization remains unclear. At present, 1,6-hexanediol (1,6-HD) is the only available tool to globally disrupt condensates, yet the conditions of 1,6-HD vary considerably between studies and may even trigger apoptosis.

Results

In this study, we first analyzed the effects of different concentrations and treatment durations of 1,6-HD and found that short-term exposure to 1.5% 1,6-HD dissolved biomolecular condensates whereas long-term exposure caused aberrant aggregation without affecting cell viability. Based on this condition, we drew a time-resolved map of 3D chromatin organization and found that short-term treatment with

SUBMITTER: Liu X 

PROVIDER: S-EPMC8369800 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature