Human Plasma Thioredoxin-80 Increases With Age and in ApoE^-/- Mice Induces Inflammation, Angiogenesis, and Atherosclerosis.

ABSTRACT:

Background

Thioredoxin (TRX)-1, a ubiquitous 12-kDa protein, exerts antioxidant and anti-inflammatory effects. In contrast, the truncated form, called TRX80, produced by macrophages induces upregulation of proinflammatory cytokines. TRX80 also promotes the differentiation of mouse peritoneal and human macrophages toward a proinflammatory M1 phenotype.

Methods

TRX1 and TRX80 plasma levels were determined with a specific ELISA. A disintegrin and metalloproteinase domain-containing protein (ADAM)-10, ADAM-17, and ADAM-10 activities were measured with SensoLyte 520 ADAM10 Activity Assay Kit, Fluorimetric, and InnoZyme TACE Activity Kit, respectively. Western immunoblots were performed with specific antibodies to ADAM-10 or ADAM-17. Angiogenesis study was evaluated in vitro with

SUBMITTER: Couchie D 

PROVIDER: S-EPMC8369893 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature