Ontology highlight
ABSTRACT:
SUBMITTER: Sarkar A
PROVIDER: S-EPMC8370014 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Sarkar Abhishek A Stephens Matthew M
Nature genetics 20210524 6
The high proportion of zeros in typical single-cell RNA sequencing datasets has led to widespread but inconsistent use of terminology such as dropout and missing data. Here, we argue that much of this terminology is unhelpful and confusing, and outline simple ideas to help to reduce confusion. These include: (1) observed single-cell RNA sequencing counts reflect both true gene expression levels and measurement error, and carefully distinguishing between these contributions helps to clarify think ...[more]