In Vivo Evolution of GES β-Lactamases Driven by Ceftazidime/Avibactam Treatment of Pseudomonas aeruginosa Infections.

ABSTRACT: The mechanisms underlying an in vivo switch in the resistance phenotype of P. aeruginosa after ceftazidime-avibactam treatment was investigated. The initial isolate (a blood culture) was resistant to meropenem but remained susceptible to antipseudomonal cephalosporins and combinations with β-lactamase inhibitors. One week after ceftazidime-avibactam therapy, a subsequent isolate (a rectal swab) recovered from the same patient showed the opposite phenotype. Whole-genome sequence analysis revealed a single SNP difference between both (ST235) isolates, leading to a P162S change in bla_GES-5, creating bla_GES-15. Thus, bla_GES-1, bla_GES-5, and bla_GES-15 were cloned and expressed in the wild-type strai

SUBMITTER: Fraile-Ribot PA 

PROVIDER: S-EPMC8370190 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature