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Application of Pulsed Electric Fields to Cancer Therapy.

ABSTRACT: This review covers the use of pulsed electric fields in cancer therapy. It is organized into three sections based on pulse length, millisecond domain, microsecond domain, and nanosecond domain. The predominant application of pulsed electric fields is the modification of the permeability of cellular membranes, sometimes referred to as electroporation. This has been used in many different ways for cancer treatment. These include introducing genes into the tumor cells to activate an immune response, introducing poisons into the tumor cells, initiating necrosis using irreversible electroporation, and initiating immunogenic cell death with nanopulse stimulation.

SUBMITTER: Nuccitelli R

PROVIDER: S-EPMC8370244 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Application of Pulsed Electric Fields to Cancer Therapy.

Nuccitelli Richard R

Bioelectricity 20190318 1

This review covers the use of pulsed electric fields in cancer therapy. It is organized into three sections based on pulse length, millisecond domain, microsecond domain, and nanosecond domain. The predominant application of pulsed electric fields is the modification of the permeability of cellular membranes, sometimes referred to as electroporation. This has been used in many different ways for cancer treatment. These include introducing genes into the tumor cells to activate an immune response ...[more]

PMID: 34471806

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Project description:Glioblastoma multiforme (GBM), is the most common form of adult malignant brain tumor, highly heterogeneous and relatively resistant to therapy with a poor prognosis. As other cancers, GBM starts from a relatively small fraction of poorly differentiated and particularly aggressive cancer stem cells (CSCs), responsible for aberrant proliferation and invasion, rapidly spreading the tumor growth in the other parts of the central nervous system. Due to the extreme tumor heterogeneity, standard therapies (i.e., surgery, chemotherapy and radiotherapy) provide poor positive outcomes and cancers usually recur. Therefore, alternative approaches, possibly targeting CSCs, are necessary for searching effective therapeutic strategies against GBM. Among many emerging new therapeutic strategies, ultra-short pulsed electric fields (PEF) are considered extremely promising in cancer therapy and our previous results demonstrated the capability of a specific electric pulse protocol, characterized by a high pulse amplitude and a short duration, to selectively affect medulloblastoma CSCs preserving normal cells. Here, we tested the same exposure protocol to investigate the response of U87 GBM cells, and of U87-derived neurospheres. By analyzing different in vitro biological endpoints and taking advantage of transcriptomic and bioinformatics analyses, we found that, independently of CSCs content, PEF exposure affected cell proliferation and differentially regulated hypoxia, inflammation and P53/cell cycle checkpoints. PEF exposure also significantly reduced the clonogenic potential, reducing the ability to form new neurospheres, and inhibited the invasion potential. Importantly, exclusively in U87 neurospheres, PEF exposure changed the expression of stemness/differentiation genes. Our results, confirm this physical stimulus as a promising treatment to destabilize the extreme intra-tumoral GBM heterogeneity opening up the possibility to develop future therapeutic strategies mediated by PEF exposure.

Dataset Information

Application of Pulsed Electric Fields to Cancer Therapy.

Publications

Application of Pulsed Electric Fields to Cancer Therapy.

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