Co-Occurrence of NDM-9 and MCR-1 in a Human Gut Colonized Escherichia coli ST1011.

ABSTRACT:

Background

The emergence of the plasmid-borne colistin-resistant gene (mcr-1) poses a great threat to human health. What is worse, the recent observations of the coexistence of mcr-1 with carbapenemase encoding genes in some bacteria caused even more concern. Yet, there is a lack of observations of such strains in the human gut.

Methods

The isolation of E. coli L889 was performed on selective medium plates. Antibiotic susceptibilities were determined by an agar dilution and a broth microdilution method. Multi-locus sequence typing (MLST) and acquired resistance genes were also characterized. Transferability of bla _NDM-9/mcr-1-carrying plasmids was determined by conjugation, replicon typing and S1-Pulsed-field gel electrophoresis

SUBMITTER: Liang G 

PROVIDER: S-EPMC8370297 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature