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Clog-free high-throughput microfluidic cell isolation with multifunctional microposts.

ABSTRACT: Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed. A swift by-passing route for non-tumor cells was integrated to prevent very common clogging problems. Performance was characterized using microbeads (10 µm) and human cancer cells that were spiked in human blood. Design-I showed a capture efficiency of 96% for microbeads and 87% for cancer cells at 1 ml/min flow rate. An improved Design-II presented a higher capture efficiency of 100% for microbeads and 96% for cancer cells. Our method of utilizing various microfluidic functions of separation, bypass and capture has successfully guaranteed highly efficient separation of rare cells from biological fluids.

SUBMITTER: Venugopal D

PROVIDER: S-EPMC8370995 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature

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Clog-free high-throughput microfluidic cell isolation with multifunctional microposts.

Venugopal Dilip D Kasani Nanda N Manjunath Yariswamy Y Li Guangfu G Kaifi Jussuf T JT Kwon Jae W JW

Scientific reports 20210817 1

Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed. A swift by-passing route for non-tumor cells was integrated to prevent very common clogging problems. Performance was characterized using microbeads (10 µm) and human cancer cells that were spiked in human bl ...[more]

PMID: 34404819

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