Dataset Information

Cloning, Heterologous Expression, and Characterization of a βκ-Carrageenase From Marine Bacterium Wenyingzhuangia funcanilytica: A Specific Enzyme for the Hybrid Carrageenan-Furcellaran.

ABSTRACT: Carrageenan is a group of important food polysaccharides with high structural heterogeneity. Furcellaran is a typical hybrid carrageenan, which contains the structure consisted of alternative β-carrageenan and κ-carrageenan motifs. Although several furcellaran-hydrolyzing enzymes have been characterized, their specificity for the glycosidic linkage was still unclear. In this study, we cloned, expressed, and characterized a novel GH16_13 furcellaran-hydrolyzing enzyme Cgbk16A_Wf from the marine bacterium Wenyingzhuangia fucanilytica CZ1127. Cgbk16A_Wf exhibited its maximum activity at 50°C and pH 6.0 and showed high thermal stability. The oligosaccharides in enzymatic products were identified by liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) and nuclear magnetic resonance (NMR) spectroscopy. It was confirmed that Cgbk16A_Wf specifically cleaves the β-1,4 linkages between β-carrageenan and κ-carrageenan motifs from non-reducing end to reducing end. Considering the structural heterogeneity of carrageenan and for the unambiguous indication of the specificity, we recommended to name the furcellaran-hydrolyzing activity represented by Cgbk16A as "βκ-carrageenase" instead of "furcellaranase".

SUBMITTER: Cao S

PROVIDER: S-EPMC8371452 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Cloning, Heterologous Expression, and Characterization of a βκ-Carrageenase From Marine Bacterium <i>Wenyingzhuangia funcanilytica</i>: A Specific Enzyme for the Hybrid Carrageenan-Furcellaran.

Cao Siqi S Zhang Yuying Y Chen Guangning G Shen Jingjing J Han Jin J Chang Yaoguang Y Xiao Hang H Xue Changhu C

Frontiers in microbiology 20210804

Carrageenan is a group of important food polysaccharides with high structural heterogeneity. Furcellaran is a typical hybrid carrageenan, which contains the structure consisted of alternative β-carrageenan and κ-carrageenan motifs. Although several furcellaran-hydrolyzing enzymes have been characterized, their specificity for the glycosidic linkage was still unclear. In this study, we cloned, expressed, and characterized a novel GH16_13 furcellaran-hydrolyzing enzyme Cgbk16A_Wf from the marine b ...[more]

PMID: 34421852

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Dataset Information

Cloning, Heterologous Expression, and Characterization of a βκ-Carrageenase From Marine Bacterium Wenyingzhuangia funcanilytica: A Specific Enzyme for the Hybrid Carrageenan-Furcellaran.

Publications

Cloning, Heterologous Expression, and Characterization of a βκ-Carrageenase From Marine Bacterium <i>Wenyingzhuangia funcanilytica</i>: A Specific Enzyme for the Hybrid Carrageenan-Furcellaran.

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