An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus.

ABSTRACT:

Background

Canine circovirus is a deadly pathogen of dogs and causes vasculitis and hemorrhagic enteritis. It causes lethal gastroenteritis in pigs, fox, and dogs. Canine circovirus genome contains two main (and opposite) transcription units which encode two open reading frames (ORFs), a replicase-associated protein (Rep) and the capsid (Cap) protein. The replicase protein and capsid protein consist of 303 amino acids and 270 amino acids respectively. Several immuno-informatics methods such as epitope screening, molecular docking, and molecular-dynamics simulations were used to craft peptide-based vaccine construct against canine circovirus.

Results

The vaccine construct was designed by joining the selected epitopes with adjuvants by suitable linker. The cloning and expressi

SUBMITTER: Jain P 

PROVIDER: S-EPMC8371590 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature