Comparison of targeted metagenomics and IS-Pro methods for analysing the lung microbiome.

ABSTRACT:

Background

Targeted metagenomics and IS-Pro method are two of the many methods that have been used to study the microbiome. The two methods target different regions of the 16 S rRNA gene. The aim of this study was to compare targeted metagenomics and IS-Pro methods for the ability to discern the microbial composition of the lung microbiome of COPD patients.

Methods

Spontaneously expectorated sputum specimens were collected from COPD patients. Bacterial DNA was extracted and used for targeted metagenomics and IS-Pro method. The analysis was performed using QIIME2 (targeted metagenomics) and IS-Pro software (IS-Pro method). Additionally, a laboratory cost per isolate and time analysis was performed for each method.

Results

Statistically significant differences were obse

SUBMITTER: Goolam Mahomed T 

PROVIDER: S-EPMC8371770 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature