Genetic variations in DNA repair gene NEIL1 associated with radiation pneumonitis risk in lung cancer patients.

ABSTRACT:

Background

Radiation pneumonitis (RP) is a common side effect in lung cancer patients who received radiotherapy. Our previous study found genetic variations in DNA repair gene NEIL1 may be a predictor of RP in patients with esophageal cancer. So, we hypothesis genetic variations in NEIL1 gene could affect the risk of RP in lung cancer patients following radiotherapy.

Methods

Genetic variations rs4462560 G>C and rs7402844 C>G in NEIL1 gene were genotyped in 174 lung cancer patients received radio(chemo)therapy. Luciferase assay, real-time PCR and Western blot were used to access the effect of the variants on NEIL1 in HELF and HEF cell lines which were transfected with plasmids containing rs4462560 G>C and rs7402844 C>G.

Results

Patients with rs4462560 CC genotype had a

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC8372061 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature