Multiplexed single-cell profiling of chromatin states at genomic loci by expansion microscopy.

ABSTRACT: Proper regulation of genome architecture and activity is essential for the development and function of multicellular organisms. Histone modifications, acting in combination, specify these activity states at individual genomic loci. However, the methods used to study these modifications often require either a large number of cells or are limited to targeting one histone mark at a time. Here, we developed a new method called Single Cell Evaluation of Post-TRanslational Epigenetic Encoding (SCEPTRE) that uses Expansion Microscopy (ExM) to visualize and quantify multiple histone modifications at non-repetitive genomic regions in single cells at a spatial resolution of ∼75 nm. Using SCEPTRE, we distinguished multiple histone modifications at a single housekeeping gene, quantified histone modifi

SUBMITTER: Woodworth MA 

PROVIDER: S-EPMC8373070 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature