Ontology highlight
ABSTRACT:
SUBMITTER: Sakamoto Y
PROVIDER: S-EPMC8373077 | biostudies-literature | 2021 Aug
REPOSITORIES: biostudies-literature
Sakamoto Yoshitaka Y Zaha Suzuko S Nagasawa Satoi S Miyake Shuhei S Kojima Yasuyuki Y Suzuki Ayako A Suzuki Yutaka Y Seki Masahide M
Nucleic acids research 20210801 14
Long-read whole-genome sequencing analysis of DNA methylation would provide useful information on the chromosomal context of gene expression regulation. Here we describe the development of a method that improves the read length generated by using the bisulfite-sequencing-based approach. In this method, we combined recently developed enzymatic base conversion, where an unmethylated cytosine (C) should be converted to thymine (T), with nanopore sequencing. After methylation-sensitive base conversi ...[more]