Single-oocyte transcriptome analysis reveals aging-associated effects influenced by life stage and calorie restriction.

ABSTRACT: Chromosome segregation errors in oocytes lead to the production of aneuploid eggs, which are the leading cause of pregnancy loss and of several congenital diseases such as Down syndrome. The frequency of chromosome segregation errors in oocytes increases with maternal age, especially at a late stage of reproductive life. How aging at various life stages affects oocytes differently remains poorly understood. In this study, we describe aging-associated changes in the transcriptome profile of mouse oocytes throughout reproductive life. Our single-oocyte comprehensive RNA sequencing using RamDA-seq revealed that oocytes undergo transcriptome changes at a late reproductive stage, whereas their surrounding cumulus cells exhibit transcriptome changes at an earlier stage. Calorie restriction, a pa

SUBMITTER: Mishina T 

PROVIDER: S-EPMC8373347 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature