Cellular carbon stress is a mediator of obesity-associated osteoarthritis development.

ABSTRACT:

Objective

'Carbon stress' is a newly found mechanism that links obesity and dysregulated metabolism. It is defined as the cellular accumulation of metabolites during obesity post-translationally modifying metabolic proteins and decreasing their enzymatic activity. The objective of this study was to investigate if 'carbon stress' also occurs in cartilage and contributes to obesity associated OA development.

Methods

We histologically evaluated for OA pathology in wild-type (WT) and hyperphagic mice (Pomc-neuron specific enhancer one deficient, Pomc^Δ1) that were subjected to standard chow (Chow, n = 6 for both genotypes) or high-fat feeding (HFD, n = 7 for both genotypes). Joints were stained and quantified for 'carbon stress' markers, including succinyl-lysine (SCK)

SUBMITTER: Liu H 

PROVIDER: S-EPMC8373780 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature