Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera.

ABSTRACT: Calcium sparks are involved in major physiological and pathological processes in vertebrate muscles but have never been characterized in invertebrates. Here, dynamic confocal imaging on intact skeletal muscle cells isolated enzymatically from the adult honey bee legs allowed the first spatio-temporal characterization of subcellular calcium release events (CREs) in an insect species. The frequency of CREs, measured in x-y time lapse series, was higher than frequencies usually described in vertebrates. Honey bee CREs had a larger spatial spread at half maximum than their vertebrate counterparts and a slightly ellipsoidal shape, two characteristics that may be related to ultrastructural features specific to invertebrate cells. In line-scan experiments, the histogram of CREs' duration followed

SUBMITTER: Collet C 

PROVIDER: S-EPMC8373864 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature