DL4-μbeads induce T cell lineage differentiation from stem cells in a stromal cell-free system.

ABSTRACT: T cells are pivotal effectors of the immune system and can be harnessed as therapeutics for regenerative medicine and cancer immunotherapy. An unmet challenge in the field is the development of a clinically relevant system that is readily scalable to generate large numbers of T-lineage cells from hematopoietic stem/progenitor cells (HSPCs). Here, we report a stromal cell-free, microbead-based approach that supports the efficient in vitro development of both human progenitor T (proT) cells and T-lineage cells from CD34⁺cells sourced from cord blood, GCSF-mobilized peripheral blood, and pluripotent stem cells (PSCs). DL4-μbeads, along with lymphopoietic cytokines, induce an ordered sequence of differentiation from CD34⁺ cells to CD34⁺CD7⁺CD5^+<

SUBMITTER: Trotman-Grant AC 

PROVIDER: S-EPMC8373879 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature