Chemotaxis in Leishmania (Viannia) braziliensis: Evaluation by the two-chamber capillary assay.

ABSTRACT: Chemotactic responses play a significant role during Leishmania (V.) braziliensis differentiation through its life cycle and during infection. The aim of this description has been to portray the modified "two-chamber capillary chemotaxis assay" as a technique useful for quantitative in vitro evaluation of Leishmania chemotaxis after reviewing the methods described until now to assess chemotaxis in vitro in Leishmania sp. This valued simple and reproducible method convenient for parasite migration determination, was tested by the use of controlled changes in monosaccharide (D-glucose and D-fructose) concentrations as referent ligands. The validation of the method demonstrates that this technique is useful to evaluate the relationship existing between paras

SUBMITTER: Lopez ED 

PROVIDER: S-EPMC8374177 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature