In situ DESI-MSI lipidomic profiles of mucosal margin of oral squamous cell carcinoma.

ABSTRACT:

Background

Although there is consensus that the optimal safe margin is ≥ 5mm, obtaining clear margins (≥5 mm) intraoperatively seems to be the major challenge. We applied a molecular diagnostic method at the lipidomic level to determine the safe surgical resection margin of OSCC by desorption electrospray ionisation mass spectrometry imaging (DESI-MSI).

Methods

By overlaying mass spectrometry images with hematoxylin-eosin staining (H&E) from 18 recruited OSCC participants, the mass spectra of all pixels across the diagnosed tumour and continuous mucosal margin regions were extracted to serve as the training and validation datasets. A Lasso regression model was used to evaluate the test performance.

Findings

By leave-one-out validation, the Lasso model achieved 88.6% a

SUBMITTER: Yang X 

PROVIDER: S-EPMC8374374 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature