An analytical method to measure the contribution of clear synaptic and dense-core peri-synaptic vesicles to neurotransmitter release from synaptic terminals with two classes of secretory vesicles.

ABSTRACT: Two types of secretory vesicles co-exist at some presynaptic terminals. Clear synaptic vesicles (CSV) release their contents at the synaptic active zone, upon single impulses, while dense-core vesicles (DCV) usually release their contents in the periphery of the terminal upon repetitive stimulation. Part of the transmitter released by DCV diffuses to produce paracrine effects, and part of it reaches the postsynaptic terminal, adding its effect to that of synaptic release. This article presents an analytical method to separate the contribution of CSV and DCV to the postsynaptic responses, based on the kinetics of postsynaptic currents (PSCs). Since stimulation with single presynaptic impulses usually triggers release only from CSV, the kinetics of the resulting PSC can be used as a template

SUBMITTER: Trueta C 

PROVIDER: S-EPMC8374493 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature