InVision: An optimized tissue clearing approach for three-dimensional imaging and analysis of intact rodent eyes.

ABSTRACT: The mouse eye is used to model central nervous system development, pathology, angiogenesis, tumorigenesis, and regenerative therapies. To facilitate the analysis of these processes, we developed an optimized tissue clearing and depigmentation protocol, termed InVision, that permits whole-eye fluorescent marker tissue imaging. We validated this method for the analysis of normal and degenerative retinal architecture, transgenic fluorescent reporter expression, immunostaining and three-dimensional volumetric (3DV) analysis of retinoblastoma and angiogenesis. We also used this method to characterize material transfer (MT), a recently described phenomenon of horizontal protein exchange that occurs between transplanted and recipient photoreceptors. 3D spatial distribution analysis of MT i

SUBMITTER: Gurdita A 

PROVIDER: S-EPMC8374524 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature