Liquid chromatography-tandem mass spectrometry analysis for identification and quantification of antimicrobial compounds in distillery wastewater.

ABSTRACT: A high-resolution mass spectrometry (HR-MS) method was developed to analyze and identify small molecule compounds in distillery wastewater. According to identification confidence levels, 4 levels of compounds were identified. The five antimicrobial compounds (lactic acid, succinic acid, acetophenone, cinnamic acid, and phenyllactic acid), which shown in high concentrations, were at the highest level of confidence (level 1, confirmed structure). Thus, a rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously quantify these antimicrobial compounds. The analysis was performed in the selective reaction monitoring (SRM) mode via the electrospray ionization (ESI) source operating in the negative ionization mode. Linear calibration curv

SUBMITTER: Hou W 

PROVIDER: S-EPMC8374650 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature