Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter.

ABSTRACT: The methylation index of the LINE-1 promoter is one of the most commonly used markers for assessing the global level of genome methylation in various human cells and tissues. We developed an NGS-based protocol for DNA methylation analysis of the LINE-1 retrotransposon promoter. This approach allows assessment of the DNA methylation index of 19 CpG sites in the LINE-1 promoter that have the highest tissue- or tumor-specific variability. The method provides a DNA methylation profile for analyzing either the methylation index of each CpG site independently or the mean DNA methylation index across the LINE-1 promoter. The results obtained using the developed method corresponded well to the level of methylation assessed using a commercially available kit for DNA pyrosequencing. In addition, our

SUBMITTER: Vasilyev SA 

PROVIDER: S-EPMC8374674 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature