Analysis of the Aqueous Humor Proteome in Patients With Age-Related Macular Degeneration.

ABSTRACT:

Purpose

Age-related macular degeneration (AMD) is associated with altered gene and protein expression in the retina. We characterize the aqueous humor (AH) proteome in AMD to gain insight into the pathogenesis of the disease and identify potential biomarkers.

Methods

AH was collected from age and gender matched neovascular AMD (nvAMD; n = 10) patients and controls (n = 10). AH was pooled to create two samples (nvAMD and control), followed by intensity-based label-free quantification (MS1). Functional and bioinformatic analysis were then performed. A validation set (20 controls, 15 atrophic AMD and 15 nvAMD) was tested via multiplex ELISA for nine differentially expressed proteins according to the MS1 findings.

Results

MS1 identified 674 proteins in the AH. 239 protein

SUBMITTER: Rinsky B 

PROVIDER: S-EPMC8374990 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature