Multi-omics of the esophageal microenvironment identifies signatures associated with progression of Barrett's esophagus.

ABSTRACT:

Background

The enrichment of Gram-negative bacteria of oral origin in the esophageal microbiome has been associated with the development of metaplasia. However, to date, no study has comprehensively assessed the relationships between the esophageal microbiome and the host.

Methods

Here, we examine the esophageal microenvironment in gastro-esophageal reflux disease and metaplasia using multi-omics strategies targeting the microbiome and host transcriptome, followed by targeted culture, comparative genomics, and host-microbial interaction studies of bacterial signatures of interest.

Results

Profiling of the host transcriptome from esophageal mucosal biopsies revealed profound changes during metaplasia. Importantly, five biomarkers showed consistent longitudinal changes

SUBMITTER: Deshpande NP 

PROVIDER: S-EPMC8375061 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature