Dataset Information

Nickel Foam Promotes Syntrophic Metabolism of Propionate and Butyrate in Anaerobic Digestion.

ABSTRACT: Enhanced interspecies electron transfer (IET) among symbiotic microorganisms is an effective method to increase the rate of methane (CH₄) production in anaerobic digestion. Direct interspecies electron transfer (DIET), which does not involve dissolved redox media, is considered an alternative and superior method to enhance methane production by interspecific hydrogen (H₂) transfer (IHT). In this study, nickel foam was built into a semicontinuous anaerobic reactor to investigate its effect on the metabolism of propionate and butyrate. Both increased the average yield of CH₄ in anaerobic digestion by 18.1 and 15.9%, respectively. Analysis of bacterial and archaeal communities showed that the addition of nickel foam could increase the relative abundance of microbial communities involved in DIET and could increase the diversity of microorganisms in the reactor. Moreover, the anaerobic digestion performance of the nickel foam reactor was good at high hydrogen partial pressure.

SUBMITTER: Li X

PROVIDER: S-EPMC8375088 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Nickel Foam Promotes Syntrophic Metabolism of Propionate and Butyrate in Anaerobic Digestion.

Li Xueyuan X Mo Haoe H Zhou Chengxuan C Ci Yuhui Y Wang Jinwei J Zang Lihua L

ACS omega 20210806 32

Enhanced interspecies electron transfer (IET) among symbiotic microorganisms is an effective method to increase the rate of methane (CH<sub>4</sub>) production in anaerobic digestion. Direct interspecies electron transfer (DIET), which does not involve dissolved redox media, is considered an alternative and superior method to enhance methane production by interspecific hydrogen (H<sub>2</sub>) transfer (IHT). In this study, nickel foam was built into a semicontinuous anaerobic reactor to investi ...[more]

PMID: 34423211

Similar Datasets

Project description:Linking the genomic content of uncultivated microbes to their metabolic functions remains a critical challenge in microbial ecology. Resolving this challenge has implications for improving our management of key microbial interactions in biotechnologies such as anaerobic digestion, which relies on slow-growing syntrophic and methanogenic communities to produce renewable methane from organic waste. In this study, we combined DNA stable-isotope probing (SIP) with genome-centric metagenomics to recover the genomes of populations enriched in 13C after growing on [13C]butyrate. Differential abundance analysis of recovered genomic bins across the SIP metagenomes identified two metagenome-assembled genomes (MAGs) that were significantly enriched in heavy [13C]DNA. Phylogenomic analysis assigned one MAG to the genus Syntrophomonas and the other MAG to the genus Methanothrix. Metabolic reconstruction of the annotated genomes showed that the Syntrophomonas genome encoded all the enzymes for beta-oxidizing butyrate, as well as several mechanisms for interspecies electron transfer via electron transfer flavoproteins, hydrogenases, and formate dehydrogenases. The Syntrophomonas genome shared low average nucleotide identity (<95%) with any cultured representative species, indicating that it is a novel species that plays a significant role in syntrophic butyrate degradation within anaerobic digesters. The Methanothrix genome contained the complete pathway for acetoclastic methanogenesis, indicating that it was enriched in 13C from syntrophic acetate transfer. This study demonstrates the potential of stable-isotope-informed genome-resolved metagenomics to identify in situ interspecies metabolic cooperation within syntrophic consortia important to anaerobic waste treatment as well as global carbon cycling.IMPORTANCE Predicting the metabolic potential and ecophysiology of mixed microbial communities remains a major challenge, especially for slow-growing anaerobes that are difficult to isolate. Unraveling the in situ metabolic activities of uncultured species may enable a more descriptive framework to model substrate transformations by microbiomes, which has broad implications for advancing the fields of biotechnology, global biogeochemistry, and human health. Here, we investigated the in situ function of mixed microbiomes by combining stable-isotope probing with metagenomics to identify the genomes of active syntrophic populations converting butyrate, a C4 fatty acid, into methane within anaerobic digesters. This approach thus moves beyond the mere presence of metabolic genes to resolve "who is doing what" by obtaining confirmatory assimilation of the labeled substrate into the DNA signature. Our findings provide a framework to further link the genomic identities of uncultured microbes with their ecological function within microbiomes driving many important biotechnological and global processes.

Dataset Information

Nickel Foam Promotes Syntrophic Metabolism of Propionate and Butyrate in Anaerobic Digestion.

Publications

Nickel Foam Promotes Syntrophic Metabolism of Propionate and Butyrate in Anaerobic Digestion.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets