Development of a model for fibroblast-led collective migration from breast cancer cell spheroids to study radiation effects on invasiveness.

ABSTRACT:

Background

Invasiveness is a major factor contributing to metastasis of tumour cells. Given the broad variety and plasticity of invasion mechanisms, assessing potential metastasis-promoting effects of irradiation for specific mechanisms is important for further understanding of potential adverse effects of radiotherapy. In fibroblast-led invasion mechanisms, fibroblasts produce tracks in the extracellular matrix in which cancer cells with epithelial traits can follow. So far, the influence of irradiation on this type of invasion mechanisms has not been assessed.

Methods

By matrix-embedding coculture spheroids consisting of breast cancer cells (MCF-7, BT474) and normal fibroblasts, we established a model for fibroblast-led invasion. To demonstrate applicability of this model,

SUBMITTER: Mei J 

PROVIDER: S-EPMC8375131 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature