Antiviral activity of diallyl trisulfide against H9N2 avian influenza virus infection in vitro and in vivo.

ABSTRACT:

Background

Diallyl trisulfide (DATS) is a garlic-derived organosulfur compound. As it has been shown to have anti-viral activity, we hypothesized that it may alleviate infections caused by H9N2 avian influenza virus (AIV), which is prevalent in poultry with pandemic potential.

Methods

Human lung A549 epithelial cells were treated with three different concentrations of DATS 24 h before (pre-treatment) or one hour after (post-treatment) H9N2 AIV infection. Culture supernatants were collected 24 h and 48 h post-infection and analyzed for viral titers and levels of inflammatory and anti-viral immune responses. For in vivo experiments, BABL/c mice were administered daily by intraperitoneal injection with DATS (30 mg/kg) for 2 weeks starting 1 day after H9N2 AIV infection. Clinica

SUBMITTER: Ming L 

PROVIDER: S-EPMC8375198 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature